名称：His Tag

别名：6 His tag; HHHHHH tag

描述

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Specification

clone：6A8H8

Host/Isotype：Mouse IgG1

Antibody Type：Tag Antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Tag

免疫原：Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Formulation：Purified antibody in PBS with 0.05% sodium azide

Application

Application：WB,IP,IHC,IF,FCM

WB：1/500-1/1000

IP：1/20-1/200

IHC：1/100 - 1/1000

ICC：1/100 - 1/1000

FCM：1/200 - 1/400

ELISA：1/10000

宿主：Mouse