名称：GST Tag

描述

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.

文献

1. 1993. Biotechnology (N Y) 11: 933-6.
2. 1988. Gene 67: 31-40.

Specification

clone：6G9C6

Host/Isotype：Mouse IgG1

Antibody Type：Tag Antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Tag

免疫原：Purified fusion protein with GST protein tag expressed in E. Coli.

Formulation：Purified antibody in PBS with 0.05% sodium azide

Application

Application：WB,IP,IF,IHC,FCM

WB：1/500 - 1/2000

IP：1/10-1/20

IHC：1/100 - 1/1000

ICC：1/100 - 1/1000

FCM：1/200 - 1/400

ELISA：1/10000

宿主：Mouse