名称：GFP Tag

描述

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

文献

1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404.
2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30.
3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68.

Specification

Swissprot：P42212

clone：4B10B2

WB Predicted band size：27kDa

Host/Isotype：Mouse IgG2a

Antibody Type：Tag Antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Tag

免疫原：Purified recombinant fragment of GFP expressed in E. Coli.

Formulation：Purified antibody in PBS with 0.05% sodium azide

Application

Application：WB,IP,IF,IHC,FCM

WB：1/500-1/1000

IP：1/20-1/50

IHC：1/200 - 1/1000

ICC：1/100-1/200

FCM：1/200 - 1/400

ELISA：1/10000

宿主：Mouse