产品名称

Anti-GFP抗体

参阅全部 GFP 一抗

描述

兔多克隆抗体to GFP

宿主

Rabbit

特异性

Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols.

GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

经测试应用

适用于: ELISA, IHC-Fr, ICC, IHC-P, IP, WB, IHC-FoFr, IHC-FrFl, Electron Microscopymore details

种属反应性

与反应: Species independent

免疫原

Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria.

Database link: P42212

阳性对照

The Recombinant A. victoria GFP protein (ab84191), any other purified recombinant GFP, any cell line confirmed to overexpress GFP. ICC: NIH3T3, U2OS and glandular stomach cells. IHC: Mouse brain and dog heart tissue. WB: Sample: COS7 and LNCaP whole cell lysate - transfected with GFP-Eml4.

常规说明

The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products).