名称：53BP1 (phospho Ser6)

别名：TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1

Modified：Phosphorylated

描述

function:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.,PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.,PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.,similarity:Contains 2 BRCT domains.,subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.,subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3.,

Specification

Entrez GeneID：7158

Swissprot：Q12888

Gene Accession：Swiss Prot:Q12888
GeneID:7158

WB Predicted band size：213kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat,Monkey

免疫原：The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser6. AA range:1-50

Formulation：Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application

Application：WB,IHC

WB：1/500 - 1/2000

IHC：1/100 - 1/300

ELISA：1/5000

宿主：Rabbit