名称：Akt1 (phospho Ser246)

别名：AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Pathway：TGF-beta signaling pathway

Pathway：PI3K-Akt signaling pathway

Pathway：mTOR signaling pathway

Pathway：MAPK signaling pathway

Pathway：Jak-STAT signaling pathway

Modified：Phosphorylated

Targets：Disease

描述

AKT serine/threonine kinase 1(AKT1) Homo sapiens The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Specification

Entrez GeneID：207

Swissprot：P31749

Gene Accession：Swiss Prot:P31749
GeneID:207

WB Predicted band size：56kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser246. AA range:212-261

Formulation：Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application

Application：WB,IHC,IF

WB：1/500 - 1/2000

IHC：1/100 - 1/300

ICC：1/200 - 1/1000

ELISA：1/5000

宿主：Rabbit