名称：BRSK1

别名：EC 2.7.11.1; SAD1 kinase; Serine/threonine kinase SAD-B;

描述

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.Lizcano J.M., EMBO J. 23:833-843(2004).Lu R., J. Biol. Chem. 279:31164-31170(2004).Nagase T., DNA Res. 8:85-95(2001).

Specification

Entrez GeneID：84446;

Swissprot：Q8TDC3

Gene Accession：Swiss-Prot: Q8TDC3NCBI Gene ID: 84446

WB Predicted band size：87kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse

免疫原：Synthesized peptide derived from internal of human BRSK1.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB

WB：1/500-1/3000

宿主：Rabbit