名称：p7S6 Kinase(Ab-424)

别名：KS6B1; P70-S6K; RPS6KB1; S6K;

Pathway：PI3K-Akt signaling pathway

Pathway：mTOR signaling pathway

描述

RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .

文献

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851

Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.

Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c

Le, X.F, et al. (2003) Oncogene 22: 484

Specification

Entrez GeneID：6198;

Swissprot：P23443

Gene Accession：Swiss-Prot: P23443NCBI Protein: NP_003152.1

WB Predicted band size：70 85 kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：Peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC,IF

WB：1/500-1/1000

IHC：1/50-1/100

ICC：1/100-1/200

宿主：Rabbit