名称：C-RAF (Phospho-Tyr341)

别名：C-RAF; C-Raf; CRAF; RAF-1;

Pathway：PI3K-Akt signaling pathway

Pathway：mTOR signaling pathway

Pathway：MAPK signaling pathway

Pathway：Jak-STAT signaling pathway

Modified：Phosphorylated

Targets：FDA approved drug target related

描述

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.David R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727. Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259. Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654. Antonino Colanzi, J. Cell Biol., Apr 2003; 161: 27

Specification

Entrez GeneID：5894;

Swissprot：P04049

Gene Accession：Swiss-Prot#: P04049; NCBI Gene#: 5894; NCBI Protein#: NP_002871.1.

WB Predicted band size：74kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Peptide sequence around phosphorylation site of tyrosine 341 (S-Y-Y(p)-W-E) derived from Human C-RAF .

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC

WB：1/500-1/1000

IHC：1/50-1/100

宿主：Rabbit