名称：eIF4G (phospho-Ser1231)

Modified：Phosphorylated

Targets：Disease

描述

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

文献

De Gregorio, E. et al. (1998) RNA 4, 828-836.

Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.

Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.

Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.

Specification

Entrez GeneID：1981;

Swissprot：Q04637

Gene Accession：Swiss-Prot: Q04637NCBI Protein: NP_004944.2

WB Predicted band size：220kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Peptide sequence around phosphorylation site of serine 1231 (P-V-S(p)-P-L) derived from Human eIF4G.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC

WB：1/500-1/1000

IHC：1/50-1/100

宿主：Rabbit