名称：NFkB-p65(Phospho-Ser536)

别名：NFKB3; RELA; TF65; Transcription factor p65; p65

Pathway：PI3K-Akt signaling pathway

Pathway：MAPK signaling pathway

Modified：Phosphorylated

Targets：Disease

描述

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

文献

Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501.

Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972.

Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.

Baeuerle P A, et al. (1996) Cell 87:13-20.

Haskill S, et al. (1991) Cell 65:1281-1289.

Specification

Entrez GeneID：5970;

Swissprot：Q04206

Gene Accession：Swiss-Prot: Q04206NCBI Protein: NP_001138610.1

WB Predicted band size：65kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：Peptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC,IF

WB：1/500-1/1000

IHC：1/50-1/100

ICC：1/100-1/200

宿主：Rabbit