名称：4E-BP1/2/3 (Phospho-Thr 45)

别名：4E-BP1 antibody
4EBP1 antibody
4EBP1_HUMAN antibody
BP 1 antibody
eIF4E binding protein 1 antibody
eIF4E-binding protein 1 antibody
Eif4ebp1 antibody
Eukaryotic translation initiation factor 4E-binding protein 1 antibody
PHAS-I antibody
PHASI antibody
Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody

Modified：Phosphorylated

Targets：FDA approved drug target related

描述

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.

Specification

Entrez GeneID：1978

Swissprot：Q13541

Gene Accession：Swiss-Prot#:O95477

WB Predicted band size：21kDa

Host/Isotype：Mouse IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human, Mouse, Rat

免疫原：peptide

Formulation：Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

Application

Application：WB,IP

WB：1/1000-1/1000

IP：1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

宿主：Mouse