名称：Paxillin(phospho-Tyr88)

别名：PAXI; PXN;

Modified：Phosphorylated

描述

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

文献

Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.

Specification

Entrez GeneID：5829;

Swissprot：P49023

Gene Accession：Swiss-Prot: P49023NCBI Protein: NP_001074324.1

WB Predicted band size：68kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IF

WB：1/500-1/1000

ICC：1/100-1/200

宿主：Rabbit