名称：eIF-2a (Phospho-Ser 52)

别名：EIF 2 alpha antibody
EIF 2 antibody
EIF 2A antibody
EIF 2alpha antibody
eIF-2-alpha antibody
eIF-2A antibody
EIF-2alpha antibody
EIF2 alpha antibody
EIF2 antibody
EIF2A antibody
EIF2S1 antibody
Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
Eukaryotic translation initiation factor 2 subunit 1 antibody
Eukaryotic translation initiation factor 2 subunit alpha antibody
IF2A_HUMAN antibody

Modified：Phosphorylated

描述

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2, eIF2 and eIF2 (eukaryotic translation initiation factor 2 , and , respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2 controls the rate of tRNA translation. When eIF2 is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Specification

Entrez GeneID：1965;

Swissprot：P05198

Gene Accession：Swiss-Prot#:P05198

WB Predicted band size：36kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human, Mouse, Rat

免疫原：A short amino acid sequence containing phosphorylated Ser 52 of eIF2α of human origin.

Formulation：Purified antibody in TBS with 0.05% sodium azide,1%BSA and 50% glycerol.

Application

Application：WB,IP,IHC

WB：1/1000-1/1000

IP：IP1-2 μg per100-500 μg of total protein (1 ml of cell lysate)

IHC：1/50-1/500

宿主：Rabbit