名称：FKHR(Phospho-Ser256)

别名：FKHR; FOXO1;

Modified：Phosphorylated

描述

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.

文献

Guo S., Rena G., Cichy S., He X., Cohen P., Unterman T.J. Biol. Chem. 274:17184-17192(1999)

Zhang X., Gan L., Pan H., Guo S., He X., Olson S.T., Mesecar A., Adam S., Unterman T.G.J. Biol. Chem. 277:45276-45284(2002)

Daitoku H., Hatta M., Matsuzaki H., Aratani S., Ohshima T., Miyagishi M., Nakajima T., Fukamizu A.Proc. Natl. Acad. Sci. U.S.A. 101:10042-10047(2004)

Specification

Entrez GeneID：2308;

Swissprot：Q12778

Gene Accession：Swiss-Prot: Q12778NCBI Protein: NP_002006.2

WB Predicted band size：70kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：A synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser256.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC,IF

WB：1/500-1/2000

IHC：1/50-1/200

ICC：1/100-1/500

宿主：Rabbit