名称：WEE1 (Phospho-Ser642)

别名：WEE1A; WEE1hu; kinase WEE1;

Modified：Phosphorylated

描述

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation

文献

Watanabe N., EMBO J. 14:1878-1891(1995)

Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001)

Igarashi M., Nature 353:80-83(1991)

Specification

Entrez GeneID：7465;

Swissprot：P30291

Gene Accession：Swiss-Prot#: P30291; NCBI Gene#: 7465; NCBI Protein#: NP_003381.1.

WB Predicted band size：100kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB

WB：1/500-1/1000

宿主：Rabbit