名称：LKB1 (Phospho-Ser428)

别名：Protein kinase; CAMK group; CAMKL family; LKB subfamily;

Pathway：PI3K-Akt signaling pathway

Modified：Phosphorylated

Targets：Disease

描述

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways.Zhu H, et al. (2013) J Biol Chem 288, 16495-505. Seto SW, et al. (2013) PLoS One 8, e66404 . Rosario FJ, et al. (2012)J Physiol 590, 1495-509.

Specification

Entrez GeneID：6794;

Swissprot：Q15831

Gene Accession：Swiss-Prot#: Q15831; NCBI Gene#: 6794; NCBI Protein#: NP_000446.1

WB Predicted band size：65kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Peptide sequence around phosphorylation site of serine428 (R-L-S(p)-A-C) derived from Human LKB1 .

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB

WB：1/500-1/1000

宿主：Rabbit