名称：IkB-a(Phospho-Ser32/Ser36)

别名：I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA

Modified：Phosphorylated

Targets：Disease

描述

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

文献

Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.Honda, R. et al. (1997) FEBS Lett. 420, 25-27.Hirao, A. et al. (2000) Science 287, 1824-1827.Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.

Specification

Entrez GeneID：4792;

Swissprot：P25963

Gene Accession：Swiss-Prot: P25963NCBI Protein: NP_065390.1

WB Predicted band size：39kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse

免疫原：Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC,IF

WB：1/500-1/1000

IHC：1/50-1/100

ICC：1/100-1/200

宿主：Rabbit