名称：Bub3 (Phospho-Tyr207)

别名：Mitotic checkpoint protein BUB3, Hbub3, BUB3L, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl

Modified：Phosphorylated

描述

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

文献

Seeley T.W., Wang L., Zhen J.Y.Biochem. Biophys. Res. Commun. 257:589-595(1999)

Tang Z., Shu H., Oncel D., Chen S., Yu H.Mol. Cell 16:387-397(2004)

Logarinho E., Resende T., Torres C., Bousbaa H.Mol. Biol. Cell 19:1798-1813(2008)

Specification

Entrez GeneID：9184;

Swissprot：O43684

Gene Accession：Swiss-Prot#: O43684 NCBI Protein#: NP_001007794.1

WB Predicted band size：40kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat

免疫原：Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB

WB：1/500-1/1000

宿主：Rabbit