名称：p53(Phospho-Ser46)

别名：P53, BCC7, LFS1, TRP53

Pathway：PI3K-Akt signaling pathway

Pathway：MAPK signaling pathway

Modified：Phosphorylated

Targets：Disease

描述

p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

文献

Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759.

Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064.

Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442.

Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959.

Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.

Specification

Entrez GeneID：7157;

Swissprot：P04637

Gene Accession：Swiss-Prot: P04637NCBI Protein: NP_000537.3

WB Predicted band size：53kDa

Host/Isotype：Rabbit IgG

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Peptide sequence around phosphorylation site of serine 46 (M-L-S(p)-P-D) derived from Human p53.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IF

WB：1/500-1/1000

ICC：1/100-1/200

宿主：Rabbit