商品详情：

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

-Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing

-Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process

-Tested and found negative for bacteria, fungi, and mycoplasma

-Protein concentrations are determined by Lowry method

-Endotoxin units are measured by Limulus Amoebocyte Lysate assay

-BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

-Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Source

Engelbreth-Holm-Swarm Mouse Tumor

Application

-Cell Growth and Differentiation

-Metabolism/Toxicology Studies

-Invasion Assays

-in vitro and in vivo Angiogenesis Assays

-in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Features

-Certified LDEV-free

-Extensive quality control exceeding industry standard

-Rigorously validated for functionality

-Most extensively references basement membrane matrix