名称：TIP60

别名：TIP60 (HTATIP)

Targets：Disease

描述

HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

文献

1. Mizuguchi, G. et al. Science 2004 303, 343-348.
2. Jin, J. et al. Trends Biochem. Sci. 2005 30, 680-687.
3. Raisner, R.M. and Madhani, H.D. Curr. Opin. Genet. 2006 Dev. 16, 119-124.

Specification

Entrez GeneID：10524

Swissprot：Q92993

clone：3F9

WB Predicted band size：60kDa

Host/Isotype：Mouse IgG2b

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Purified recombinant fragment of human TIP60 expressed in E. Coli.

Formulation：Ascitic fluid containing 0.03% sodium azide.

Application

Application：IHC

IHC：1/200 - 1/1000

ELISA：1/10000

宿主：Mouse