名称：MPL

别名：MPLV; TPOR; C-MPL; CD110

Targets：FDA approved drug target related

描述

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

文献

Cancer Res. 2009 Apr 15;69(8):3681-8.
J Biol Chem. 2009 May 1;284(18):11781-91.

Specification

Entrez GeneID：4352

Swissprot：P40238

clone：1H2

WB Predicted band size：71.2kDa

Host/Isotype：Mouse IgG2b

Antibody Type：Primary antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human

免疫原：Purified recombinant fragment of human MPL expressed in E. Coli.

Formulation：Purified antibody in PBS with 0.05% sodium azide

Application

Application：FCM

FCM：1/200 - 1/400

ELISA：1/10000

宿主：Mouse