名称：GAPDH

别名：G3PD; GAPD; MGC88685;Glyceraldehyde-3-phosphate dehydrogenase

Targets：FDA approved drug target related

描述

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer

文献

1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
2. Sumner CJ. Ann Neurol 2003.54:6 47-54.

Specification

Entrez GeneID：2597

Swissprot：P04406

clone：1A10

WB Predicted band size：37kDa

Host/Isotype：Mouse IgG1

Antibody Type：Loading Control Antibody

Storage：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity：Human,Mouse,Rat,Rabbit,Monkey,Pig

免疫原：Purified recombinant fragment of human GAPDH expressed in E. Coli.

Formulation：Purified antibody in PBS with 0.05% sodium azide.

Application

Application：WB,IHC,IF,FCM

WB：1/5000- 1/20000

IHC：1/200 - 1/1000

ICC：1/200 - 1/1000

FCM：1/200 - 1/400

ELISA：1/10000

宿主：Mouse