Activity: Recombinant Human Ubiquitin-AMC is a fluorogenic substrate for some Ubiquitin-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nm and an emission wavelength of 445 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-AMC concentration of 0.1-1 μM.

Source: E. coli-derived human Ubiquitin protein

Accession #: P0CG47.1

Predicted Molecular Mass: 8.7 kDa