简述

For purification of total RNA from cells, tissues, and yeast

描述

 Fast procedure delivering high-quality total RNA in minutes Ready-to-use RNA for high performance in any downstream application Consistent RNA yields from small to large amounts of starting material No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation High-throughput processing in 96-well format and automatable protocols RNeasy Kits are the Gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica-membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured-cell samples using silica-membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect.

性能

RNeasy Kits deliver highly reproducible yields of total RNA from small to large samples. Total RNA can reliably be purified from small numbers of cells, including a single cell, as well as from small amounts of standard tissues (see figures "Reliable RNA isolation from a single cell", "Highly reproducible yields for sensitive applications" and "High-quality total RNA from fine needle aspirates") using the RNeasy Micro Kit. RNA purified using the RNeasy Micro Kit provides maximum sensitivity with precious samples in quantitative gene expression analyses, such as real-time RT-PCR, by efficient on-column digestion of genomic DNA (see figure "Efficient on-column removal of genomic DNA").

Total RNA is easily purified from animal or human cells, animal or human tissues, and yeast (see table “Total RNA yields obtained with RNeasy Kits” and figure "RT-PCR of RNA from as few as 100 cells").