英文名称 Anti-AF-6（B-5） Monoclonal Antibody 靶标 ARRB1 别名 AF 6; AF6; AFAD_HUMAN; Afadin; ALL1-fused gene from chromosome 6 protein; FLJ34371; MLLT 4; MLLT4; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4; Myeloid/lymphoid or mixed lineage leukemia translocated to 4; Protein AF 6; Protein Af-6; Protein AF6; RP3 431P23.3; 5033403D15Rik; Gm314; I-afadin; Mllt4; S-afadin. 宿主 Rabbit 稀释比例 WB?1:100- 1:1000. IP 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate).?IF ?1:50-1:500. IHC 1:50-1:500. ELISA 1:30-1:3000 Gene ID 4301 保存 Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Swiss Prot P55196 蛋白分子量 207kDa 储存液 PBS with < 0.1% sodium azide and 0.1% gelatin. 亚细胞定位 Cell membrane 免疫原 Recombinant fusion protein of Human AF-6 亚型 IgG2aK 纯化方法 Affinity purification 克隆号 B-5 背景资料 The dynamics of a cell-cell interface such as tight junctions or adherensjunctions are important in many developmental, physiological and pathological processes. AF-6 (MLLT4: myeloid/lymphoid or mixed-lineage leukemia translocated to 4) is a 1,612 amino acid protein that contains two N-terminal Ras binding domains (RBD) and a GLGF motif, and is implicated in Ras-mediated signaling events occurring at peripheral cell-cell junctions. AF-6 interacts with F-Actin and Profilin in cell-cell junctions, and may modulate Actin modeling near adhesion complexes. Furthermore, AF-6 coordinates junction adhesion molecule (JAM) recruitment to intercellular junctions through a PDZ domain. Developing mice deficient in AF-6 activity display a loss of neuroepithelial polarity, suggesting that AF-6 activity is an important regulator of cell-cell junctions that influence apical/basolateral asymmetry 克隆类型 Monoclonal antibody 交叉反应 Human Rat Mouse 应用 WB ELISA IHC-P IF IP 规格 1ml 使用前请离心。如有问题请及时联系我们。