英文名称 Anti-SMURF2 Polyclonal Antibody 靶标 SMU1 别名 E3 ubiquitin-protein ligase SMURF2; EC 6.3.2.; hSMURF2; MGC138150; Smad specific E3 ubiquitin ligase 2; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMUF2_HUMAN; Smurf2; Ubiquitin protein ligase SMURF2; 宿主 Rabbit 稀释比例 IHC 1:50-200. Gene ID 64750 保存 Store at -20°C. Avoid freeze / thaw cycles. Swiss Prot Q9HAU4 蛋白分子量 86kDa 储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. 亚细胞定位 Cytoplasm Nucleus 免疫原 A synthetic peptide of SMURF2 亚型 IgG 纯化方法 Octanoic acid-ammonium sulfate precipitation 背景资料 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed:30696809). Public Immunogen Range 155-205/748aa 克隆类型 Polyclonal Antibody 交叉反应 Human Mouse 应用 IHC 规格 50ul 100ul 使用前请离心。如有问题请及时联系我们。