英文名称 Anti-NUDT16 Polyclonal Antibody 靶标 NUDT16 别名 Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme 宿主 Rabbit 稀释比例 IHC 1:50-200. Gene ID 131870 保存 Store at -20°C. Avoid freeze / thaw cycles. Swiss Prot Q96DE0 蛋白分子量 18/21/22/25kDa 储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. 亚细胞定位 Nucleus Cytoplasm 免疫原 Recombinant protein of human NUDT16 亚型 IgG 纯化方法 Affinity purification 背景资料 RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596). Public Immunogen Range 34-193aa 克隆类型 Polyclonal Antibody 交叉反应 Human Predicted:Bovine Chicken Sheep Mouse 应用 IHC 规格 50ul 100ul 使用前请离心。如有问题请及时联系我们。