英文名称 Anti-PD-L1/CD274 Polyclonal Antibody 靶标 PD-L1/CD274 宿主 Rabbit 稀释比例 WB 1:500-1000. IHC 1:300-1200.IF 1:10-100.IP 0.5-4.0 ug for IP and 1:500-2000 for WB Gene ID 29126 保存 Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. Swiss Prot Q9NZQ7 蛋白分子量 33kDa 储存液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3. 亚细胞定位 Cell membrane 免疫原 Fusion protein of human PD-L1/CD274 亚型 IgG 纯化方法 Affinity purification 背景资料 Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058). 克隆类型 Polyclonal Antibody 交叉反应 Human Rat Mouse 应用 WB ELISA IP IF IHC 规格 50ul 100ul 使用前请离心。如有问题请及时联系我们。