靶标 PAM 别名 PAL;PHM 宿主 Rabbit 稀释比例 IHC 1:100-300. Gene ID 5066 保存 Store at -20°C. Avoid freeze / thaw cycles. Swiss Prot P19021 蛋白分子量 96/100/108kDa 储存液 Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. 亚细胞定位 Cell membrane Secreted 免疫原 Recombinant protein of human PAM 亚型 IgG 纯化方法 Affinity purification 背景资料 Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate. Public Immunogen Range 1-350aa 克隆类型 Polyclonal Antibody 交叉反应 Human Rat Mouse 应用 IHC 规格 50ul 100ul 使用前请离心。如有问题请及时联系我们。