基本信息(General Information)：

Name (Chinese) 分散酶II

Name (English) Dispase II

Specifications Reagent grade, ≥0.5units/mg, protease

Chemical Formula

Synonym (Chinese) 蛋白酶 来源于多粘芽孢杆菌

Synonym (English) Protease from Bacillus polymyxa

Beilstein Registry No. -

EINECS Number 255-914-4

MDL Number MFCD02253017

UNSPSC Code 12352204

产品描述(Description)：

Analysis Note The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival

Application The enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso

Biochem/physiol Actions Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes.

Physical form lyophilized powder containing calcium acetate and milk sugar

Preparation Note A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

性质(Properties)：

activity ≥0.5 units/mg solid

solubility 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble

storage temp. 2-8°C

安全信息(Safety Information)：

Hazard Pictogram Codes GHS07, GHS08

Signal Word Danger

Hazard Statements H315-H319-H334-H335

Precautionary Statements P261-P305 + P351 + P338-P342 + P311

Personal Protective Equipment -

Hazard Codes (Europe) Xn

Risk Codes (Europe) 36/37/38-42

Safety Codes (Europe) 22-24-26-36/37

RIDADR NONH for all modes of transport

WGK Germany 3

RTECS -

Flash Point (F) -

Flash Point (C) -