单位定义

One unit is defined as the amount of enzyme required to give 50% ligation of 100 ng HindIII fragments of λ DNA in a total reaction volume of 20 μl in 30 minutes at 25°C in 1X T7 DNA Ligase Reaction Buffer.

反应条件

1X StickTogether™ DNA Ligase Buffer

Incubate at 25°C

1X StickTogether™ DNA Ligase Buffer

66 mM Tris-HCl

10 mM MgCl2

1 mM ATP

1 mM DTT

7.5% Polyethylene glycol (PEG 6000)

(pH 7.6 @ 25°C)

贮存溶液

10 mM Tris-HCl

50 mM KCl

1 mM DTT

0.1 mM EDTA

50% Glycerol

pH 7.4 @ 25°C

热失活

否