DNA Endonuclease

Catalyzes the cleavage of DNA mismatches and non-β DNA structures including Holliday junctions and cruciform leaving 3'-OH and 5'-phosphate

Best at C mismatches and does not recognize all DNA mismatches

To a lesser extent cleaves across a nick in dsDNA

Key enzyme for use in genome editing mutation detection workflows

单位定义

One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C.

反应条件

1X NEBuffer™ 2

Incubate at 37°C

1X NEBuffer™ 2

50 mM NaCl

10 mM Tris-HCl

10 mM MgCl2

1 mM DTT

(pH 7.9 @ 25°C)

贮存溶液

20 mM Tris-HCl

200 mM NaCl

1 mM DTT

0.1 mM EDTA

50% Glycerol

0.15% Triton® X-100

pH 7.5 @ 25°C

热失活

否