Hybond N+ is a positively charged nylon membrane capable of high sensitivity in DNA and RNA blotting. Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems Recommended for all high density membrane arrays Amersham Hybond-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation.